2 x Laemmli Sample buffer

0.125 M Tris/HCl            1.25 ml of 2 M Tris/HCl buffer pH 6.8
4                                   SDS 8 ml of 10 SDS
20 Glycerol                   4.6 ml of 87 Glycerol
10-mercaptoethanol   2 ml
H₂O                                4.15 ml
                          In total 20 ml

Add some (1-2 mg) bromphenol blue.
Store frozen in small aliquots.

Alternatively, make buffer without -mercaptoethanol and store at room temperature. Add -mercaptoethanol just before using.

Chemicals and solutions:

• 87 Glycerol, ‘Merck’, No. 1.04094

• 2 M Tris/HCl pH 6.8: 24.22 g/100 ml H₂O

2004 02 05 – Julius