GEL ELECTROPHORESIS OF PROTEINS
- Resolving and stacking gel solutions for SDS-PAGE (18.5 cm x 20 cm gels).
- Resolving and stacking gel solutions for SDS-PAGE (7.5 cm x 10 cm gels).
- Laemmli sample and running buffers for SDS-PAGE.
- The extraction of mitochondrial proteins and the chemicals and solutions for isoelectric focusing.
- Separation of mitochondrial integral membrane proteins from soluble and membrane-associated proteins by salt extraction and carbonate wash.
- The composition of IEF sample solution.
- Preparation of samples for SDS-PAGE by precipitation with trichloroacetic acid (TCA) and acetone.
- Blue Native polyacrylamide gel electrophoresis: BN-PAGE.
- Silver, Coomassie and SYPRO Ruby staining and destaining of proteins in polyacrylamide gels.
- In-gel alkylation and digestion of proteins with endoproteinase Glu-C (V8) and modified trypsin.