Anthocyanins as substrates for mitochondrial complex I - protective effect against heart ischemic injury

Anthocyanins, a subclass of flavonoids, are known to protect against myocardial ischemia, however, little is known about their direct, acute effects on mitochondria injured by the ischemic insult. In this study, the effects of delphinidin 3-O-glucoside (Dp3G), cyanidin 3-O-glucoside (Cy3G) and pelargonidin 3-O-glucoside (Pg3G) on the activity of complex I of the mitochondrial respiratory chain were studied in mitochondria isolated from normal and 45 min ischemia-damaged rat hearts. Cy3G and Dp3G increased the activity of complex I in ischemia-damaged mitochondria measured in the presence or absence of CoQ1 whereas in non-ischemic mitochondria the effect was observed only in the absence of CoQ1 . Dp3G and Cy3G but not Pg3G increased State 3 respiration and ATP synthesis with NADH-dependent substrates in mitochondria after ischemia. The results suggest that certain anthocyanins can act as electron acceptors at complex I and bypass ischemia-induced inhibition resulting in increased ATP production after ischemia. This study provides new information on a possible application of certain anthocyanins in the regulation of energy metabolism in mammalian cells.